p creb ser133 87g3  (Cell Signaling Technology Inc)

Journal: Breast cancer research : BCR

Article Title: Transcriptomic analysis identifies enrichment of cAMP/PKA/CREB signaling in invasive lobular breast cancer.

doi: 10.1186/s13058-024-01900-y

Figure Lengend Snippet: Fig. 5 A Dose response curves representing growth inhibition in ILC (red: SUM44PE, MM134, MM330, WCRC25, MM453, HCC2185) and NST (blue: MCF7, T47D, MM415, BT474) cell lines in response to treatment with forskolin: Y axis represents fold change in growth after 7 days of treatment with forskolin and X axis represents log of dose of forskolin in µM B Overlapping box and jitter plot depicting IC50 (half maximal inhibitory concentration) for forskolin in ILC (red: SUM44PE, MM134, MM330, WCRC25, MM453, HCC2185) and NST (blue: MCF7, T47D, MM415, BT474) cell lines; C ILC—Invasive lobular breast cancer; NST—Invasive carcinoma—no special type; PKACA—Protein kinase A catalytic α subunit; CREB—cAMP Response Element Binding protein; p-CREB—phospho CREB, phosphorylated at Ser133; Ecad KO—E-cadherin Knock Out; IC50—Half maximal inhibitory concentration

Article Snippet: Primary antibody incubation was performed overnight at 4 °C: P-CREB (Ser133) (87G3) (Cell Signaling Technology #9198; 10 ug/ml), PKA-Cα (Cell Signaling Technology #4782; 1:100).

Techniques: Inhibition, Concentration Assay, Binding Assay, Knock-Out

Journal: Journal of Nanobiotechnology

Article Title: Conversion of nanoscale topographical information of cluster-assembled zirconia surfaces into mechanotransductive events promotes neuronal differentiation

doi: 10.1186/s12951-016-0171-3

Figure Lengend Snippet: The interaction with the neuritogenesis-inducing surface has an impact on transcription factor dynamics relevant for neuronal differentiation. a The confocal images show the average stack projection of p-CREB stainings of cells in the indicated experimental conditions. The cells were fixed with 4 % PFA 60 min or 24 h after in the indicated conditions and stained for the nucleus (HOECHST), f-actin and p-CREB. The outer dashed lines represent the outlines of the cells obtained from the f-actin staining and the inner dashed line the nuclear area determined from the HOECHST staining. The graph (representing the global statistics of two independent experiments, n = 33–76 cells) summarizes the corresponding quantification performed with the help of ImageJ (see also “ ”). b The same experimental procedure and quantification (from three independent experiments, n: > 500 cells, >150 neurites) as in Additional file : Figure S2A–C, but with an inhibitor against JNK/c-jun (SP600125 10 and 20 µM). Representative images of the conditions can be found in Additional file : Figure S3

Article Snippet: Antibodies or fluorescence reagents: 4B4 (Beckman Coulter) and K20 (Santa Cruz Biotechnology, Santa Cruz, USA, California) against β1 integrin, 87G3 antibody against p-CREB (Cell signaling, Danvers, USA, Massachusetts), hVin-1 antibody against vinculin (Sigma-Aldrich), HOECHST 33342 (Molecular Probes (Thermo Fischer Scientific), Waltham, USA, Massachusetts), TRITC-Phalloidin (Sigma-Aldrich).

Techniques: Staining